ANALYSIS METHOD OF L-HYDROXYPROLINE, MEASUREMENT METHOD OF COLLAGEN, AND D-HYDROXYPROLINE DEHYDROGENASE

摘要

PROBLEM TO BE SOLVED: To provide a novel analysis method capable of simply analyzing L-hydroxyproline.SOLUTION: An analysis method of analyzing L-hydroxyproline in a test sample comprises: (s1) an isomerization step of isomerizing L-hydroxyproline in the test sample to D-hydroxyproline with L-hydroxyproline isomerase; (s2) a dehydrogenation step of dehydrogenating D-hydroxyproline obtained by the step (s1) with D-hydroxyproline dehydrogenase; and (s3) an analysis step of analyzing the dehydrogenation performed by the step (s2).