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New iron / zinc binding regulators and their regulation to improve iron deficiency tolerance and promote iron / zinc accumulation in edible parts

机译:新型铁/锌结合调节剂及其调节剂,可提高对缺铁的耐受性并促进可食部分中铁/锌的积累

摘要

According to the present invention, a transformant and a gene-disrupted strain are provided which exhibit growth superior to that of ordinary plants in calcareous soil, and which can accumulate iron and zinc in large quantities in both calcareous soil and good soil; also provided are a gene, vector, protein, and antibody used for constructing these, and a method of construction, a composition for construction, a kit for construction, and a breeding method for a plant with improved iron deficiency tolerance, and enhanced iron and zinc accumulation in an edible part. The protein of the present invention is an iron- and zinc-binding regulatory factor, and includes any one of the following amino acid sequences of (a) to (c): (a) an amino acid sequence represented by SEQ ID NO:1 or 2; (b) an amino acid sequence obtained by deletion, substitution, or addition of one to several amino acids in the amino acid sequence represented by SEQ ID NO:1 or 2; or (c) an amino acid sequence which has 80% or more identity with the amino acid sequence represented by SEQ ID NO:1 or 2.
机译:根据本发明,提供了在钙质土壤中表现出优于普通植物生长的转化体和基因破坏株,并且可以在钙质土壤和良好土壤中均积累大量铁和锌。还提供了用于构建这些基因,载体,蛋白质和抗体的基因,载体,蛋白质和抗体,以及具有改善的铁缺乏耐受性和增强的铁和铁的植物的构建方法,构建组合物,构建试剂盒和育种方法。锌在可食用部分中积累。本发明的蛋白质是铁和锌结合的调节因子,并且包括以下(a)至(c)的任何氨基酸序列:(a)由SEQ ID NO:1表示的氨基酸序列。或2; (b)通过在SEQ ID NO:1或2表示的氨基酸序列中缺失,取代或添加一个至几个氨基酸而获得的氨基酸序列; (c)与SEQ ID NO:1或2表示的氨基酸序列具有80%或更多同一性的氨基酸序列。

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