Method for genetic inactivation of unfertilized puffer eggs, method for producing super male pufferfish having sex chromosome YY, and method for producing male pufferfish using the same

摘要

PPROBLEM TO BE SOLVED: To provide a technique capable of surely obtaining supermale globefishes having YY sex chromosomes and thereby finally and surely producing only the male globefishes. PSOLUTION: The super male globefishes 11 having YY sex chromosomes can be obtained through the steps of radiation exposure for genetically inactivating eggs by applying a radiation dose 3 of 50 to 800 Gy to the unfertilized eggs 1 of the globefish, fertilizing the genetically inactivated unfertilized eggs 4 with sperm 6 of a normal male globefish 5, and giving heat shock 8 to haploid embryos 7 to block the second segmentation, and performing the polyploidization of the chromosome. By mating the super male globefish 11 with a normal female globefish 12, only the male globefishes 15 can be produced. As the globefish, Takifugu rubripes or Takifugu porphyreus is preferable. PCOPYRIGHT: (C)2011,JPO&INPIT