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Method for producing transgenic imperfectly transformed insect, method for producing transgenic imperfectly transformed insect egg, and transgenic imperfectly transformed insect

机译:转基因不完全转化昆虫的生产方法,转基因不完全转化昆虫卵的生产方法和转基因不完全转化昆虫

摘要

PPROBLEM TO BE SOLVED: To provide a method for making a transgenic hemimetabolous insect, by which the transgenic individuals of the hemimetabolous insect can efficiently be made. PSOLUTION: The method for making the transgenic hemimetabolous insect is characterized by comprising a site-specifying process for specifying the site of primordial germ cells in the egg of the hemimetabolous insect; an injection process for injecting transposase mRNA and a transposon having an arbitrary DNA inserted thereinto into a place near to the specified site in the egg of the hemimetabolous insect; and a selection process for selecting the transgenic bodies each having the arbitrary DNA inserted thereinto, from hemimetabolous insects born from the injected eggs. PCOPYRIGHT: (C)2011,JPO&INPIT
机译:

要解决的问题:提供一种制备转基因半代谢昆虫的方法,通过该方法可以有效地制备该半代谢昆虫的转基因个体。

解决方案:所述转基因半代谢昆虫的制备方法的特征在于,包括用于确定所述半代谢昆虫的卵中的原始生殖细胞的位点的定点过程;用于将转座酶mRNA和具有插入其中的任意DNA的转座子注射到半代谢昆虫卵的指定位置附近的注射过程;以及从从注入的卵中生出的半代谢昆虫中选择各自插入有任意DNA的转基因体的选择方法。

版权:(C)2011,日本特许厅&INPIT

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