Production method of the transformation earthworm which utilizes the reproduction nest playback ability of the earthworm, production method null of the rearrangement protein 1) after cutting off the first portion which includes the reproduction nest of the earthworm

摘要

Transformation earthworms, and the present invention relates to a method for producing a recombinant protein from body fluids of transformed earthworms manufacturing method of transformation earthworm using gonad regeneration ability of the earthworm, has been prepared by this. Preparation Methods of transformation worms according to the invention, reproduction fibroblasts has a high injection efficiency of the new biotechnological techniques form to complement the disadvantages of transformation prior art, having totipotency, such as embryonic stem cells Through the use, it is possible to insert recombinant genes in any area of ​​the transformants, it is possible to produce a recombinant protein through the body fluid of the transformant, it is directly fed to the target gene in the gonad With, for surrogate (animal) is not required, use reproductive transformants, it is possible to produce a large number of transformants continuously, and simpler than transforming existing technology, and , it does not require skilled techniques and expensive equipment.