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Nucleosides and oligonucleotides for studies on reversal of cytotoxic and mutagenic damage of DNA potential diagnostics tools
Nucleosides and oligonucleotides for studies on reversal of cytotoxic and mutagenic damage of DNA potential diagnostics tools
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机译:核苷和寡核苷酸用于研究DNA潜在的细胞毒性和诱变破坏的逆转诊断工具
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摘要
This invention relates to n-alkylated synthetic nucleosides and phosphoramidites of high regio-specific purity and stability and for selective deprotection of the protecting group in oligonucleotides for the purpose of synthesis of high purity selectively n-alkylated sequence specific DNA and RNA. Such oligonucleotides are useful for study of mechanism of cytotoxic and mutagenic DNA damage, detection and reversal of cellular cytotoxic and mutagenic damages that occurs from the incorporation of methylated nucleosides, the corresponding phosphates and triphosphates and their precursors, via de novo DNA synthesis. The reagents could be extremely valuable tools as diagnostics and mutagenic reversal reagents. The present invention describes N-1-nitrogen alkylated purine and N-3 nitrogen alkylated pyrimidines with appropriate nucleobase amino protection, which are selectively deprotected with minimal migration of the alkyl substituent under the condition which are required for synthesizing high purity regiospecific N-1 and N-3 nitrogen alkylated oligonucleotides. A novel process has been developed to synthesize N-3 nitrogen alkylated cytidine.
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