Method for designing heat-resistant tyrosine-dependent short-chain dehydrogenase/reductase and heat-resistant tyrosine-dependent short-chain dehydrogenase/reductase

摘要

A method for designing a heat-resistant mutant enzyme, the method including the step of reducing a distance between the α4 helix and the α6 helix in a protein three-dimensional structure to become smaller than that of a wild type enzyme through deletion, replacement, addition, or insertion of one or several amino acids in the amino acid sequence of the wild type enzyme with respect to tyrosine-dependent short-chain dehydrogenase/reductase.