METHOD FOR DETECTING DIFFERENTIATING AND QUANTIFYING T CELL POPULATIONS BY MEANS OF REVERSE TRANSCRIPTION QUANTITATIVE REAL TIME PCR (RT-QPCR) TECHNOLOGY

摘要

The invention relates to a method for detecting differentiating and quantifying T cell populations comprising the following steps: a) bringing a first aliquot of a bodily fluid of an individual in contact with at least one antigen wherein the bodily fluid contains antigen presenting cells (APCs) and T cells b) incubating the first aliquot with the at least one antigen over a certain time period c) detecting and differentiating the T cell populations by detecting at least one first marker of the APCs induced by the T cells of a certain T cell population in the first aliquot and in a second aliquot of the bodily fluid of the individual which was not incubated with the at least one antigen by means of reverse transcription quantitative real time polymerase chain reaction (RT qPCR) and d) detecting and quantifying the T cell populations by determining the ratio of the detected markers of the APCs of the first aliquot to the second aliquot. The invention further relates to a kit for carrying out the method.