METHOD OF DETERMINING OF SUBSTANCES OF DYTERPENOVYH GLYCOSIDES IN STEVIA LEAVES (STEVIA REBAUDIANA BERTONI)

摘要

Method of determining of substances of dyterpenovyh glycosides in stevia leaves (Stevia rebandiana Bertoni) is to obtain the extract by extracting 1 g crushed to particle size of 0.7-1.2 mm leaf Stevia 20 ml of 95% ethanol in a boiling water bath for 15 reverse refrigerators min., its cooling, application of 0.01 ml of extract and 1% solution of a standard sample in methanol glycoside to the start line of the plate for TLC micropipette or syringe, air drying plates for 10 min., chromatography ascending manner in the chamber with solvent system chloroform-methanol-water as 60-30-6, drying the plate in air for 30 min., spraying plate 50% sulfuric acid, drying in the oven at 100 ° C for 15 minutes. indentyfikuvanni spots and calculation glycosides. Chopped leaf stevia extraction is carried out with purified water at a temperature of 75-80 ° C for 50-65 minutes.