NATURAL CRYOPRESERVATIVE AGENT FOR BONE MARROW-DERIVED STEM CELLS AND CRYOPRESERVATION METHOD FOR BONE MARROW-DERIVED STEM CELLS USING SAME

摘要

The present invention relates to a cryopreservative agent for bone marrow-derived stem cells, and the present invention is achieved by the steps of: preparing a cryopreservative agent of various compositions of DMSO, FBS, trehalose, catalase and zVAD-fmk; cryopreserving for three weeks a bone marrow-derived mesenchymal stem cell introduced into the cryopreservative agent; after thawing, measuring and evaluating the cell viability and cell proliferation; and measuring the expression of a specific surface marker and stem cell marker according to the conditions, and by confirming and evaluating the differentiation ability after inducing the myocyte differentiation according to the conditions, where the present invention has an effect of providing a cryopreservative agent which protects cells when subjecting bone marrow-derived mesenchymal stem cells to cryopreservation, allows a long-term cryopreservation of stem cells and exhibits excellent effects such as maintaining high viability and achieving differentiation into tissue-specific cells.