METHODOLOGY FOR CONSTRUCTION OF ULTRA-DENSITY GENETIC MAP BY HIGH-THROUGHPUT SEQUENCE-BASED SNP GENOTYPING

摘要

The present invention relates to a kind of ultra dense degree of genetic map for building to use the breeding population of the SNP genotype based on next-generation sequencing. According to the accuracy of this method, developing can be verified using SNP genotype next generations sequencing (NGS), in silicon because utilizing biological characteristics propagating population, the not experiment of additive. In addition, the size for the chromosome that the algorithm that method includes exploitation is generated in view of characteristic is by across being more than the RIL offsprings, or by hybridization obtain F2 in maternal and male parent. When method of the invention be used for 100,000 to 1,000,000 SNP marker is located at chromosome and tests without Genotyping, and density is caused to increase, so that providing has in the ultra dense degree of high-resolution genetic map 3 months. ;The 2014 of copyright KIPO submissions;[Reference numerals] (AA) sequence and generate next-generation sequencing equipment; (BB) rough draft gene; (CC) it is directed at (BWA); SNP (DD) dives (extraction of SAM tools); (EE) SNP is filtered; (FF) it is selected between SNP, in maternal and male parent; (GG) consensus sequence extracts (SAM tools); (HH) filling missing is sequentially connected; Prepare the parent and RIL agreements of SNP sequences (II) matrix; At deletion (JJ) than being 10%; (KK, RR) does not have; (LL, SS) " is "; The offspring's block for analyzing SNP genotype (MM) pattern is based on biological heredity aspect; (NN) recognize that the two sides of pattern delete website and delete website; (OO) prepare desirable genotype matrix; (PP) prepare that map is added; (QQ) reproducibility is verified; (TT) Map Integration is added; Prepare a kind of ultra dense degree genetic map (UU)