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Expression and purification method of biological active human FGF2 with human PDIb´a´ domain in Escherichia coli
Expression and purification method of biological active human FGF2 with human PDIb´a´ domain in Escherichia coli
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机译:具有人PDIb´a´结构域的生物活性人FGF2的表达和纯化方法在大肠杆菌中
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摘要
The present invention provides methods of E. coli in human FGF2 expression and purification of recombinant proteins using human PDIb'a' domain relates to a human protein disulfide isomerization enzyme (human protein disulfide isomerase; hPDI) gene or the gene of any one gene encoding a domain hPDIb'a' ; And the human fibroblast growth factor 2 (human fibroblast growth factor; hFGF2) provides a recombinant expression vector containing the gene . Also provides a recombinant microorganism transformed with the conversion the recombinant expression vector , the recombinant protein provides hFGF2 (rhFGF2) produced by the production method and the production method of the hFGF2 recombinant protein (rhFGF2). ; 展开▼