RECOMBINANT STRAIN of Escherichia of coli - PRODUCER OF HYDROLASE OF THE ESTER OF ALPHAAMINO ACIDS FROM Xanthomonas of rubrilineanS AND METHOD FOR MICROBIOLOGICAL SYNTHESIS OF HYDROLASE OF THE ESTER OF ALPHAAMINO ACIDS ON TO THE BASIS OF THIS STRAIN

摘要

FIELD: biotechnologies.;SUBSTANCE: strain is built by transformation of strain-recipient Escherichia coli BL21 (DE3) with plasmid DNA containing the coding area of gene aehR of hydrolase of alpha-amino acids esters Xanthomonas rubrilineans All-Russian collection of industrial microorganisms B-9915 under control of promoter T7 and gene of stability to kanamycin Kan of hydrolase of alpha amino acids esters from Xanthomonas rubrilineans All-Russian collection of industrial microorganisms B-9915. The proposed synthesis method of hydrolase of alpha-amino acids esters is implemented by cultivation of strain-producer Escherichia coli All-Russian collection of industrial microorganisms B-11246 with addition to the composition of environment of kanamycin and isopropyl-"в"-D-tiogallactoside as an expression inducer of the obtained product.;EFFECT: high biosynthesis level of target product, which can be used for development of methods for obtaining biocatalysts of synthesis processes of aminopenicillins and aminocephalosporins.;2 cl, 3 dwg, 1 tbl, 5 ex