A method for diagnosing atopic dermatitis in children

摘要

1. A method for diagnosis of atopic dermatitis in children, comprising mixing and cooling the patient's blood sample to the temperature of melting ice, precipitation and isolation of the erythrocytes, washing the washing medium at the temperature of melting ice, packaged preincubation (deposited) in erythrocytes predinkubirovaniya medium at 37 ° C for 3 hours, the precipitation of the resulting slurry, removing the outer lithium transference packed red blood cells in the medium free of sodium, in medium B, rich in sodium, in each of these media was incubated within at the expression period, 37 ° C with occasional shaking, and then selects an equal number of the erythrocyte suspension in the two environments, their separate precipitation, then gaining the supernatant of each sample diluted with water to produce a measurement of the lithium content in both media, on the obtained concentration lithium determine the rate of sodium in the lithium countertransport V as the difference between the concentration of lithium in an environment rich in sodium, and a medium free of sodium, in formuleV = (ANa-AMg) · K M lithium / 1 l cells / h, where ANa - lithium concentration in an environment rich in sodium; AMg - lithium concentration in an environment rich in magnesium (free from sodium) and K - coefficient associated with dilution media in the previous steps, a value of speed sodium-lithium countertransport membrane erythrocyte exceeding 258 lithium uM / l cells / h, diagnose atopic dermatitis in detey.2. A method according to claim 1, characterized in that the precipitation of the erythrocytes is carried out by centrifugation of 3000 g for 5 mode min.3. A method according to claim 1, characterized in that the separation of erythrocytes and plasma is carried