The recombinant DNA encoding the mature fibroblast growth factor RIGHTS (hFGF21), recombinant plasmid pAS010213, provides a synthesis of hFGF21 CELLS Escherichia coli, and a strain of E. coli BL21 (DE3) / pAS010213 - PRODUCER OF RECOMBINANT hFGF21

摘要

1. A recombinant DNA encoding mature fibroblast growth factor 21, human (hFGF21), the nucleotide sequence (SEQ ID №1) which is optimized for protein production in the cell of Escherichia coli.2. The recombinant plasmid pAS010213, containing NdeI / XhoI DNA fragment with the nucleotide sequence SEQ ID №1, encoding a mature polypeptide hFGF21, and NdeI / XhoI fragment of plasmid rET23a; recombinant plasmid pAS010213 provides a high level of expression in cells hFGF21 Escherichia coli.3. The recombinant strain E. coli BL21 (DE3) / pAS010213 - producing hFGF21 protein.;1。编码成熟成纤维细胞生长因子21，人（hFGF21）和核苷酸序列（SEQ ID_1）的重组DNA，其被优化用于大肠杆菌细胞中的蛋白质生产。重组质粒pAS010213含有NdeI/XhoI DNA片段，核苷酸序列SEQ ID_1，编码成熟多肽hFGF21和质粒rET23a的NdeI/XhoI片段，重组质粒pAS010213在大肠杆菌hFGF21细胞中高效表达。重组大肠杆菌BL21（DE3）/PAS010213产生HFGF21蛋白。