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RECOMBINANT PSEUDO-ADENOVIRUS PARTICLE BASED ON GENOME OF HUMAN ADENOVIRUS OF SEROTYPE 5, PRODUCING INFLUENZA HEMAGGLUTININ OF STRAIN A/Brisbane/59/2007(H1N1) AND METHOD OF ITS USE
RECOMBINANT PSEUDO-ADENOVIRUS PARTICLE BASED ON GENOME OF HUMAN ADENOVIRUS OF SEROTYPE 5, PRODUCING INFLUENZA HEMAGGLUTININ OF STRAIN A/Brisbane/59/2007(H1N1) AND METHOD OF ITS USE
FIELD: biotechnology.;SUBSTANCE: recombinant pseudo-adenovirus particle based on the genome of human adenovirus of serotype 5 and the method of its use are characterised. The presented particle comprises an expressing cassette with the insert of the hemagglutinin gene of influenza virus, at that the hemagglutinin gene of influenza virus was used as hemagglutinin of strain A/Brisbane/59/2007(H1N1) with the nucleotide sequence preliminary optimised for expression in human cells, presented in SEQ ID N0:2. The said haemagglutinin gene of influenza virus is cloned into an expression cassette comprising the signal of polyadenylation SV40 under control of the cytomegalovirus promoter. The presented invention can be used to induce specific immunity against influenza virus of A subtype H1 and H5 when administered to a subject in an effective amount, by providing enhanced expression of the recombinant hemagglutinin of influenza virus A/Brisbane/59/2007 (H1N1).;EFFECT: increased efficiency of connection use.;6 cl, 9 dwg, 1 tbl, 4 ex
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机译：领域：生物技术;目的：表征基于血清型5人腺病毒基因组的重组伪腺病毒颗粒及其使用方法。提出的颗粒包括带有流感病毒血凝素基因插入片段的表达盒，其中流感病毒的血凝素基因被用作菌株A / Brisbane / 59/2007（H1N1）的血凝素，其核苷酸序列已针对表达进行了初步优化SEQ ID NO：2所示的人细胞中的抗性。将所述流感病毒的血凝素基因克隆到表达盒中，该表达盒在巨细胞病毒启动子的控制下包含聚腺苷酸化SV40的信号。当以有效量施用于受试者时，通过提供流感病毒A /布里斯班/ 59/2007（H1N1）的重组血凝素的增强表达，本发明可用于诱导针对H1亚型和H5亚型的流感病毒的特异性免疫。 。;效果：提高连接使用效率。; 6 cl，9 dwg，1 tbl，4 ex
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