首页> 外国专利> Nucleotide primer set for LAMP amplification, used for the arteriviruses, the method of amplifying and / or detecting said virus using the same

Nucleotide primer set for LAMP amplification, used for the arteriviruses, the method of amplifying and / or detecting said virus using the same

机译:用于小动脉病毒的用于LAMP扩增的核苷酸引物组,使用该引物对所述病毒进行扩增和/或检测的方法

摘要

PROBLEM TO BE SOLVED: To provide a primer set for specifically and rapidly amplifying a nucleic acid derived from Arterivirus, and a method for amplifying and/or detecting the virus using the same.SOLUTION: There is disclosed a nucleic acid primer set for LAMP amplification used for specifically amplifying a nucleic acid derived from Arterivirus, wherein the primer set contains at least one polynucleotide selected from a group consisting of polynucleotides respectively indicated by a FIP primer, a BIP primer, a F3 primer and a B3 primer. There is further disclosed a method for determining the presence and absence of the existence of Arterivirus by using a reaction product produced by the LAMP amplification and a specific probe group immobilized on a substrate.
机译:解决的问题:提供用于特异性和快速扩增源自小动脉病毒的核酸的引物组,以及使用该引物组扩增和/或检测该病毒的方法。解决方案:公开了用于LAMP扩增的核酸引物组。用于特异性扩增衍生自动脉病毒的核酸的核酸,其中所述引物组包含至少一种多核苷酸,所述多核苷酸选自分别由FIP引物,BIP引物,F3引物和B3引物表示的多核苷酸。还公开了一种通过使用由LAMP扩增产生的反应产物和固定在基质上的特异性探针基团来确定是否存在动脉病毒的方法。

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