GENOME CAPTURE AND SEQUENCING TO DETERMINE GENOME-WIDE COPY NUMBER VARIATION

摘要

Provided herein is a capture library for target enrichment of sequences of interest from a genome DNA sample. The capture library comprises a plurality of capture oligos tiling a plurality of capture regions evenly-spaced along a genome. Each two adjacent capture regions of the plurality capture regions are separated by a spacing of about 6 to about 14 kilobases in length. The plurality of capture regions has a size of about 150 base pairs in length. Further, each capture oligo of the plurality of capture oligos comprises average 70 nucleotides in length. The capture libraries are suitable for enriching about 150 base pairs region approximately every 10 kilobases in a genome DNA. This capture library can be used to measure replication timing and copy number variation in human pediatric acute lymphocytic leukemia samples, and is also broadly applicable to any CNV application.