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METHOD OF SNP DETECTION BY USING GENE DETECTION TECHNIQUE IN BEAD-BASED MICROFLUIDICS
METHOD OF SNP DETECTION BY USING GENE DETECTION TECHNIQUE IN BEAD-BASED MICROFLUIDICS
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机译:基于基因检测技术的基于珠粒的微流体SNP检测方法
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摘要
The present invention provides a method of SNP detection by using gene detection in bead-based microfluidics comprising following steps: (a) preparing a microbead with a duplex DNA; (b) inserting a dye into the duplex DNA; (c) delivering the microbead into a microchannel with a temperature gradient region; (d) heating the temperature gradient region to denature the duplex DNA; (e) monitoring a fluorescence intensity of the duplex DNA during the step (d) to obtain a melting curve; and (f) determining the SNP by a melting curve analysis method; wherein the duplex DNA is synthesized by a target single-strand DNA and an allele-specific probe. Furthermore, the present invention offers a temperature gradient region to enhance the measurement accuracy. The continuous-flow mechanism in this region is validated and optimized.
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