首页> 外国专利> 'Nucleic Acid Test Method on a Stand, Nucleic Acid Test Kit on a Stand, Use of a Labeled Oligonucleotide Complementary to a Single-Base Nucleotide Stretch for Testing the Condition of Nucleic Acids Immobilized on a Solid Stand' by heat or light cross-linking or chemical immobilization and method for nucleic acid analysis '

'Nucleic Acid Test Method on a Stand, Nucleic Acid Test Kit on a Stand, Use of a Labeled Oligonucleotide Complementary to a Single-Base Nucleotide Stretch for Testing the Condition of Nucleic Acids Immobilized on a Solid Stand' by heat or light cross-linking or chemical immobilization and method for nucleic acid analysis '

机译:通过热或光交联法“在架子上进行核酸测试方法,在架子上进行核酸测试套件,使用与单碱基核苷酸延伸序列互补的标记寡核苷酸来测试固定在固体架子上的核酸的状况”或化学固定和核酸分析方法”

摘要

The present invention relates to a method for testing nucleic acids on a support, comprising the immobilization of one or more nucleic acids via crosslinking, wherein each of the immobilized nucleic acids includes a stretch of nucleotides of only one basetype, the provision of labeled oligonucleotides complementary to said stretch of nucleotides, and the determination of a value indicative for the condition of said nucleic acids. The present invention further relates to a kit for testing nucleic acids on a support comprising an array of nucleic acids immobilized on a solid support, wherein each of the immobilized nucleic acids includes a stretch of nucleotides of only one basetype and a labeled oligonucleotide complementary to said stretch of nucleotides. The invention additionally concerns the use of a labeled oligonucleotide complementary to a stretch of nucleotides of only one basetype for testing the condition of nucleic acids immobilized on a solid support.
机译:本发明涉及一种用于测试支持物上的核酸的方法,该方法包括通过交联固定一个或多个核酸,其中每个固定的核酸仅包含一个碱基类型的核苷酸片段,提供标记的寡核苷酸互补所述核苷酸区段的“末端”,并确定指示所述核酸状况的值。本发明进一步涉及用于测试支持物上的核酸的试剂盒,其包含固定在固体支持物上的核酸阵列,其中每个固定的核酸包括仅一个碱基型的一段核苷酸和与所述核酸互补的标记的寡核苷酸。核苷酸序列。本发明还涉及与仅一种碱基类型的核苷酸的片段互补的标记寡核苷酸在测试固定在固体支持物上的核酸的状况中的用途。

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