Use of Salmonella enterica ssp. enterica serovar Typhimurium antigens for serological differentiation of infected and vaccinated pigs

摘要

In the present invention, there is described a method of serological differentiation of pigs infected by Salmonella enterica ssp. enterica sérovar Typhimurium from pigs vaccinated with a vaccine based on an inactivated culture of Salmonella enterica ssp. enterica sérovar Typhimurium. The invention is characterized in that using the ELISA or westernblot method a level of antibodies against at least one native or recombinant protein being selected from the group consisting of SseB, Gene ID: 1252916; SipB, Gene ID: 1254408 and SipD, Gene ID: 1254406, and at least one native or recombinant protein being selected from the group consisting of FliC, Gene ID: 1253480 and YfgL, Gene ID: 1254042, is determined in a serum sample. The infected animals are identified in such a manner that if compared with vaccinated animals they have a higher level of antibodies against the SseB or SipB or SipD protein and at the same time, they have a lower level of antibodies against the FliC or YfgL protein. On the other hand, the vaccinated animals are identified in such a manner that if compared with infected animals, they have a higher level of antibodies against the FliC or YfgL protein and at the same time a lower level of antibodies against the SseB or SipB or SipD protein. The serological differentiation of infected and vaccinated pigs is necessary for selecting therapeutic processes resulting in the reduction of percentage of the Salmonella positive slaughter pigs. Another reason for the necessity of differentiation of infected and vaccinated animals is the exclusion of a false positivism of the vaccinated pigs when assessing contamination of the piggeries with Salmonella ssp. Using so far known serological methods in some Western European countries.