In-house elisa for ferritin as marker for assessing bioavailability of iron using caco-2 cell line

摘要

This invention relates to a process for the assessment of bioavailability of iron in test foods comprising the steps of culturing Caco-2 cells in 6-well plate in a medium to obtain differentiated Caco-2 cells, subjecting test foods by simulated in vitro gastric digestion followed by in vitro intestinal digestion to obtain an intestinal digest, replacing the medium in the transwell plates with fresh medium adding the intestinal digest in an insert provided with a dialysis membrane and inserting the insert into the wells of the 6-well plates, subjecting the plate to incubation followed by removal of the digest and a step of further incubation, harvesting the cells, lysing the cells to obtain a cell lysate, followed by estimating cellular ferritin content in the cell lysate using a ELISA kit.