'ANTIBODY BASED PIEZOELECTRIC BIOSENSOR FOR DETECTION OF HEPATITIS- B'

摘要

The present investigation describes the construction of an antibody- based piezoelectric biosensor capable of detecting Hepatitis B antigen in diluted cultures of attenuated Hepatitis B virus. In this context different sizes of At-cut quartz crystals are biomodified and XRD and Scanning Electron Microscopy was done to check for surface modifications. This was done to check the suitability of the crystals for use in fabrication of immunosensor. It is well known that the resonating frequency of an oscillating piezoelectric crystal can be affected by a change in the mass at the crystal surface. The piezoelectric immunosensors can measure the small change in mass. Gold thin flim was made on the AT cut quartz crystal electrode by sputter coating and Antibody control and test(Hepatitis) were immobilized onto the crystal which was pre-coated with protein A. The change in frequency was recorded after each step. The results are promising. It is an inexpensive, accurate, and reliable method for detection of Hepatitis B. it is most useful immunosensor from society & medical point of view.