Effective method of extraction and separation of antioxidant molecules from lichens obtained from kolli hills of tamil nadu

摘要

Lichens are made by symbiotic association of both fungí and algae. The fungi vvas found to be the major partner and ¡n the metabolite production too. The lichen metabolites were found to be very iinique compared with the other plant kingdom. Most of the lichen compounds were observed as very good antioxidants for capturing the free radicáis. Keep in this mind, present invention deals about the novel approaches for extraction and separation of antioxidant molecules from various lichen species. Macrolichen species were collected from different altitudes from the Eastern Ghats covering kolli hills of Tamil Nadu. The collected samples were stored at -80°C for further use. lt was subjected to extract the antioxidant compounds by keeping the fresh thalli at 62 - 65° C for about 10 minutes. Then lichen thallus was shade dried for 2-3 days under room temperature. Fine powder vvas obtained by using a mixed grinder with 250 RPM for 15minutes. An equal.proportion of ethyl acétate and methanol vvas added to the powder for the liquid- liquid extraction. Aqueous top layer vvas treated with IN NaOH and then the soíution was acidified to pH 2.5 by using IN HCI. Centrifuge the sample and discards the pellet. Supernatant was transferred into dialysis process for 72 hrs. Then the sample was air dried and analyzed for their antioxidant potential. Concentrated sample vvas spotted on the TLC píate and active constitutes were observed as different colour"in TLC píate. TLC píate showed that number of spots were reduced which interns helps to purify the antioxidant molecule very easily. Further HPLC analysis was contlrmed the separation of antioxidant compounds in an effective way by obtaining various peaks.