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Monoclonal antibody specific for shigella flexneri and process for preparation thereof

机译:对弗氏志贺氏菌具有特异性的单克隆抗体及其制备方法

摘要

The present invention provides for a monoclonal antibody specific for Shigella flexneri and process for preparation thereof. The present invention discloses a monoclonal antibody generated against whole cell lysate of Shigella flexneri and a method of generating the same, wherein the monoclonal antibody comprises a heavy chain comprising a region as set forth in SEQ ID NO: 1 or a part thereof, and a light chain comprising a region as set forth in SEQ ID NO: 2 or a part thereof. The present invention also provides for dot ELISA method for specific detection of Shigella flexneri in contaminated food, environmental and/or clinical samples using the monoclonal antibody specific for Shigella flexneri.
机译:本发明提供对弗氏志贺氏菌具有特异性的单克隆抗体及其制备方法。本发明公开了针对弗氏志贺氏菌全细胞裂解物产生的单克隆抗体及其产生方法,其中所述单克隆抗体包含重链,所述重链包含SEQ ID NO:1所示的区域或其部分,以及轻链,其包含如SEQ ID NO:2所示的区域或其一部分。本发明还提供了点ELISA方法,该方法使用对弗氏志贺氏菌具有特异性的单克隆抗体来特异性检测受污染的食物,环境和/或临床样品中的弗氏志贺氏菌。

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