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Bioassays with quality Controlled cell-based medicinal products and Nutraceuticals

机译:具有质量控制的基于细胞的药品和保健食品的生物测定

摘要

Refers to a method for determining the potency of inhibition of translation initiation of a composition with an unknown degree of inhibition activity of translation initiation comprising: a) contacting a cell with a number of access ATCC PTA 13010 with said composition For a time and at a temperature effective to inhibit the Proliferation of the cell; (b) toContact with a cell number of access ATCC PTA 13011 with said composition for a time and at a temperature effective to inhibit the Proliferation of the cell; (c) to measure the level of inhibition of Cell Proliferation of the number of access with ATCC PTA - 13010 and the cell number of Acces Or ATCC PTA 13011 induced by Said composition; (d) comparing the level of inhibition of proliferation InducGone for the composition with the level of inhibition of proliferation induced by a standard with a known amount of such activity; (e) identify the composition
机译:指用于确定具有未知水平的翻译起始抑制活性的组合物的翻译起始抑制能力的方法,该方法包括:a)使具有许多可及性ATCC PTA 13010的细胞与所述组合物接触一段时间和有效抑制细胞增殖的温度; (b)在有效抑制细胞增殖的时间和温度下,与具有所述组合物的进入编号为ATCC PTA 13011的细胞接触; (c)测量由所述组合物诱导的ATCC PTA-13010的进入次数和Acces或ATCC PTA 13011的细胞数对细胞增殖的抑制水平; (d)将组合物的InducGone抑制增殖水平与具有已知活性的标准品诱导的抑制增殖水平进行比较; (e)确定组成

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