USE OF GRB2, GATA3, IGM, AND MAVS GENES TO DETERMINE SUSCEPTIBLE PHENOTYPES AND RESISTANCE TO INFECTIOUS SALMON ANEMIA (ISA) IN ATLANTIC SALMON (SALMO SALAR)

摘要

Using genes selected from the group consisting of Grb2, Gata3,IgM, MAVS and Regul genenes that serve as predictors to determinesusceptible and resistant phenotypes to infectious salmon anemia(ISA) in Atlantic salmon (Salmo salar), quantifying the profileexpression of these genes using PCR analysis in real time, andwherein a sample with the expression pattern for a defiance bycohabitation with ISA, which has no significant difference whencompared to the expression pattern of these genes in a controlsample without defiance by cohabitation to ISA, is indicative ofISA resistant phenotype, namely, which has as a characteristic,less than 40% mortality for the ISA virus and heritability ofsaid character greater than or equal to 0.35; and a sample havinga pattern of expression to a defiance of cohabitation to ISAwhich happens to be low when compared to a control sample withoutdefiance by cohabitation to ISA, is indicative of a susceptiblephenotype to ISA, namely, presented as characteristic , amortality rate exceeding 50% for the ISA virus and a heritabilityof such minor or equal to 0.34.