Genetic marker linked to genetic sex of yellowtail, sex discrimination method for yellowtail, and primer for use in sex discrimination method

摘要

Genomic DNA from a yellowtail is subjected to a PCR method or a nucleic acid amplification reaction method using an yellowtail-derived nucleic acid as a template, wherein the PCR method or the nucleic acid amplification reaction method is carried out using, as primers, oligonucleotides comprising the nucleotide sequences: 5'-TTTCATTGTGGCGCTCAG-3' and 5'-GGTTGTAATGTGTCCCAG-3' both of which are produced based on a gene marker for genetic linkage group (LG) 12 in an yellowtail genetic linkage map. A product of the method is subjected to a gel electrophoresis method to analyze the product. In this manner, the genetic sex of the yellowtail can be determined. This technique enables the determination of the genetic sex of an immature yellowtail with reliability without killing the yellowtail.