ANALYSIS METHOD membrane-bound hemoglobin in erythrocytes by spectroscopy Raman GIANT ON nanostructured coatings

摘要

1. A method for the analysis of membrane-bound hemoglobin in erythrocytes using Raman scattering spectroscopy (SERS), characterized in that it is used for the study of nanostructured coatings in the form of annular silver nanostructures with a hierarchical structure, wherein the rims silver rings consist of communicating with each other porous aggregates micron sized silver on the surface of which are arranged and embedded in a matrix of spherical silver nanoparticles size nm.2 2-100. A method according to claim 1, characterized in that the nanostructured coatings with controlled morphology do not cause hemolysis of red blood cells and allow them to non-invasive diagnosis method GKR.3. A method according to claim 1, characterized in that the immobilization time cells on nanostructured coatings is from 5 to 40 minut.4. The method of claim 1, wherein the SERS spectra were obtained using a green laser of wavelength 514 or 532 nm.