RECOMBINANT PLASMID of pESAT6-CFP10-DBD, THE RECOMBINANT STRAIN of Escherichia of coli M15 OF PREP4, pESAT6-CFP10-DBD, METHOD OF OBTAINING, IMMOBILIZATION, CONTENT AND CLEANING RECOMBINANT PROTEIN ESAT6-CFP10-DBD HUMS DEXTRAN, RECOMBINANT PROTEIN ESAT6-CFP10-DBD AND THE IMMUNOGENIC COMPOSITION, WHICH CONTAINS PROTEIN ESAT6-CFP10-DBD

摘要

FIELD: medicine, pharmaceutics.;SUBSTANCE: group of inventions refers to gene engineering, biochemistry, biotechnology and immunology, and represents recombinant plasmid pESAT6-CFP10-DBD, recombinant strain Escherichia coli M15 [pREP4, pESAT6-CFP10-DBD], a method for preparing, immobilising, concentrating and purifying recombinant protein ESAT6-CFP10-DBD on dextran, recombinant protein ESAT6-CFP10-DBD with molecular weight 26.4 kDa, an immunogenic composition containing protein ESAT6-CFP10-DBD immobilised on dextran, specifically activating T-lymphocytes synthesising IFN-gamma with antigen stimulation of mycobacteria.;EFFECT: invention enables ensuring the high level of protein expression, which induces the immune response to mycobacterial antigen effectively.;5 cl, 2 dwg, 1 tbl, 5 ex