Recombinant plasmid DNA pcDNA4-Apo-2NLS2, CARRIER PROTEIN synthetic gene apoptin induces p53 INDEPENDENT tumor cell apoptosis

摘要

A recombinant DNA plasmid pcDNA4-Apo-2NLS2, synthetic carrier protein apoptin gene with twice NLS2 site and intended for the expression of the apoptin protein, selectively inducing apoptosis of neoplastic p53 independent human tumor cells with the nucleotide sequence SEQ ID NO1 size of 393 bp, represented by FIG. 4, having a molecular weight of 5.13 MDa size 7896 bp and comprising, in accordance with the physical and genetic map of FIG. 5: - operon size 1071 bp consisting of the early-late promoter, CMV, leader fragment peptide sequence penetrant HIV TAT protein apoptin gene and the chicken anemia virus epitope sequence Flag; - promoter CMV 232-819 bp .; - 863-882 bp T7 promoter; - Xpres primer 1112-1130 bp; - TOPO cloning site 1184 bp; - genetic markers:... the resistance gene Zeocin; b-lactamase gene that determines the resistance to ampicillin; - unique restriction sites: ClaI (1918); XbaI (2619); BamHI (2993); SalI (3001); BstWI (3425).