A method for quantifying anthocyanins in herbal drugs

摘要

Method quantification of anthocyanins in the medicinal plant material by spectrophotometry, characterized in that the method is carried out by extracting anthocyanins from medicinal plants hydroalcoholic mixtures containing 0.5-1.0% hydrochloric acid, with the ethyl alcohol concentration of not less than 70% and quantifying the content of anthocyanins in optical density of 95% ethanol containing 0.5% ammonia, against the reference solution when the analytical wavelength of maximum absorption in the range 611-630 nm using a standard sample tsiandinina-3-O-glucoside solution or using the value of its specific absorption index of 95% ethanol containing 0.5% ammonia.