METHOD OF QUALITATIVE DIFFERENTIAL EXPRESS-DIAGNOSTICS OF LIP MUCOSA NEOPLASMS BY CONTENT OF BIOMARKERS IN PATIENT'S ORAL FLUID

摘要

FIELD: medicine.;SUBSTANCE: invention relates to field of medicine, namely to medical diagnostics, and describes method of qualitative differential diagnostics of benign and malignant neoplasms of lip mucosa by content of biomarkers in patient's oral fluid. Method includes sampling patient's oral fluid, centrifugation of patient's oral fluid at 3000 rev/min for 15 minutes, dilution of oral fluid with physiological solution in ratio 1:100, re-centrifugation at 3000 rev/min for 15 minutes, placement of ready for analysis material of patient's oral fluid into pan and carrying out standard method of enzyme immunoassay on die on the basis of monoclonal antibodies. As independent biomarkers selected are matrix metalloproteinase 8/matrix metalloproteinase 8 (MMP 8/MMP 8) and metalloproteinase 9/matrix metalloproteinase 9 (MMP 9/MMP 9). Method is characterised by high accuracy and sensitivity of differential express-diagnostics of benign and malignant neoplasms of lip mucosa, simplification of patient's preparation for sampling and storage of diagnostic material, possibility of obtaining result on the day of patient's visit, high probability of identification of benign and malignant neoplasms in patient's organism both at early and at later stages of the disease.;EFFECT: invention can be used in differential diagnostics of benign and malignant neoplasms of oral cavity organs under conditions of stomatological, oncologic and surgical medical institutions.;3 cl, 6 ex