CELL OF MYCELIAL FUNGUS Penicillium canescens - XYLANASE AND LACCASE PRODUCENT, METHOD OF OBTAINING COMBINED EXYME XYLANASE AND LACCASE PREPARATION

摘要

FIELD: medicine, pharmaceutics.;SUBSTANCE: group of inventions relates to biotechnology. Claimed is cell of mycelial fungus Penicillium canescens with removed catabolite repression and arabinose induction, producing xylanase and laccase. Cell is transformed with plasmid, containing promoter of gene bgaS, bgaS leader peptide, xylanase-coding DNA fragment, bgaS terminator, β-lactamase gene and pMB1 replicon, and plasmid, which contains promoter of gene bgaS, bgaS leader peptide, laccase-coding DNA fragment, bgaS terminator, β-lactamase gene and pMB1 replicon. Also claimed is method of enzyme preparation of xylanase and laccase with application of said cell of Penicillium canescens.;EFFECT: group of inventions provides increased output of target enzymes.;10 cl, 9 dwg, 3 tbl, 4 ex