SET OF REAGENTS AND METHOD FOR DETECTING DNA OF CAUSATIVE AGENTS OF PLAGUE, ANTHRAX AND TULAREMIA BY PCR METHOD WITH HYBRIDISATION-FLUORESCENT RECORDING OF RESULTS

摘要

FIELD: biotechnology.;SUBSTANCE: set comprises six species-specific oligonucleotide primers and three probes, complementary to DNA fragments of genes of Yersinia pestis, Bacillus anthracis and Francisella tularensis. The DNA targets for specific amplification is a fragment of chromosomal gene uro2088 methyltransferase of Yersinia pestis, a fragment of chromosomal gene sspE of Bacillus anthracis, and a fragment of chromosomal DNA of insertion element ISFtu5 of Francisella tularensis.;EFFECT: invention enables to detect fast and highly specifically these strains, regardless of the plasmid profile of the strains.;2 cl, 3 dwg, 5 tbl, 2 ex