METHOD OF DIFFERENTIATION OF TOXIGENIC GENETICALLY MODIFIED STRAINS VIBRIO CHOLERAE OF BIOVAR EL TOR WITH DIFFERENT EPIDEMIC POTENTIAL BY METHOD OF MULTIPLEX POLYMERASE CHAIN REACTION AND TEST SYSTEM FOR ITS IMPLEMENTATION

摘要

FIELD: biotechnology.SUBSTANCE: characterised method comprises carrying out of PCR with use of specific primers to genes vc0497, vc0502 and vc0514 from the island of pandemicity VSP-II. The characterised test system comprises the components for isolation of DNA, the components for carrying out PCR, including, in particular, a primer mix VSPIIreg-F - 5'-TGGAAAGAAGAGCGTTACTGC-3', VSPIIreg-R - 5'-CCCTGTTGATGATGTGATTTG-3' to the gene vc0497, VSPIIpilin-F - 5'-CTGTGATTCGGGCTTTATCGG-3', VSPIIpilin-R - 5'-GCGTAAACTGAGCCAATAAGC-3' to the gene vc0502, VSPIIchem-F - 5'-CTTGATGGAGCGGAGAAAAC-3', VSPIIchem-R - 5'-CGATGAATAGCCTGTTGAAC-3' to the gene vc0514, taken in the ratio 1:1:1:1:1:1, respectively.EFFECT: inventions enable to differentiate quickly and reliably the toxigenic genetically modified strains to genovariants with low and high epidemic potential.2 cl, 1 dwg, 2 tbl