COMBINATION CRYOPROTECTOR 'DIMETHYL SULPHOXIDE/RHEOPOLYGLUCIN' FOR STEM CELL CRYOPRESERVATION AND METHOD FOR CRYOPRESERVATION THEREOF FOR CLINICAL APPLICATIONS

摘要

FIELD: medicine.;SUBSTANCE: method for stem cell cryopreservation involves preparing a stem cell suspension and adding a cryoprotector solution to the above suspension with constant stirring, which is followed by cryopreservation, wherein the cyoprotector added to the suspension is 50% DMSO in rheopolyglucin to the final DMSO concentration of 10% in the prepared suspension. The group of inventions also concerns a combination cryoprotector for stem cell cryopreservation, containing 50% DMSO in rheopolyglucin.;EFFECT: creating the cryoprotector with a lower molecular weight and lower DMSO concentration for improving post-thawing cell preservation.;2 cl, 1 ex, 5 tbl