Method and master - mix for the quantitative real-time pcr - - - target nucleic acid molecules for multiplexing

摘要

The present invention relates to two a method and a master - mix for detecting and quantifying of at least two target - nucleic acid molecules within the same multiplex - amplification reaction with the use of a einkanaldetektors in a fluorescence - detection system.It was the object of the invention, a simple and less expensive method and / or a master - mix of the invention is to provide and / or the a quantitative multiplexing of at least two target - nucleic acid molecules within the same multiplex - amplification reaction to enable.The object is achieved by means of two a method for detecting and quantifying of at least two target - nucleic acid molecules within the same multiplex - amplification reaction with the use of a single-channel in a fluorescence after detection system, by means of a master - mix for the detection and quantification of at least two target - nucleic acid molecules within the same multiplex - amplification reaction, as well as by a process for the production of a reaction mixture for use in the case of the detection and quantification of at least two target - nucleic acid molecules within the same multiplex - amplification reaction.