FLUORESCENCE MICROSCOPE FOR MULTI-FLUORESCENCE IMAGE OBSERVATION, FLUORESCENCE IMAGE OBSERVATION METHOD USING THE SAME, AND MULTI-FLUORESCENCE IMAGE OBSERVATION SYSTEM

摘要

A fluorescent microscope for observing multiple fluorescent images includes: a first optical module comprising a first light source for supplying first excitation light having a first wavelength, a first excitation filter for selectively transmitting the first excitation light supplied from the first light source, a first dichroic filter for reflecting the first excitation light having passed through the first excitation filter toward the survey object, an objective lens for condensing the first excitation light reflected by the first dichroic filter and transferring the condensed first excitation light to the survey object, a second dichroic filter for reflecting first radiation light radiated from the survey object, a first radiation filter for selectively transmitting the first radiation light reflected by the second dichroic filter, and a first image acquisition unit for acquiring an image by using the first radiation light having passed through the first radiation filter to be supplied; and a second optical module comprising a second light source for supplying second excitation light having a second wavelength, a second excitation filter for selectively transmitting the second excitation light supplied from the second light source, a second radiation filter passing through the second excitation filter and irradiated to the survey object to be radiated, to selectively transmitting the second radiation light EM2 having passed through the objective lens, the first dichroic filter, and the second excitation filter, and a second image acquisition unit for acquiring an image by using the second radiation light having passed through the second radiation filter to be supplied.