ESCHERICHIA COLI VARIANT, EXTRACT, ACELLULAR PROTEIN SYNTHESIS REACTION SOLUTION, STABLE ISOTOPE-LABELED PROTEIN SYNTHESIS KIT, AND PRODUCTION METHOD OF STABLE ISOTOPE-LABELED PROTEIN

摘要

PROBLEM TO BE SOLVED: To provide an extract for simply synthesizing a strictly stable isotope-labeled protein, an acellular protein synthesis reaction solution, a stable isotope-labeled protein synthesis kit, and a production method of the stable-isotope labeled protein, as well as an Escherichia coli cell body used for them.SOLUTION: The Escherichia coli variant of the invention is characterized by that the enzymatic activity of two or more metabolic enzymes selected from the group consisting of asparaginase, serine deaminase, glutamine synthetase, γ-glutamyl cysteine synthetase, aspartate ammonia lyase, asparagine synthetase, arginine decarboxylase, and tryptophanase is lowered or inactivated.SELECTED DRAWING: None