Provided are an enzyme immobilization electrode capable of easily immobilizing an enzyme while retaining activity, an electrode for production of an enzyme immobilization electrode which is suitably used for production of the enzyme immobilization electrode, and a biofuel cell using the enzyme immobilization electrode. In a biofuel cell having a structure in which a positive electrode and a negative electrode face each other with a proton conductor interposed therebetween, and configured to extract an electrode from a fuel using an enzyme, an electrode which includes a mixture containing carbon particles and a water-insoluble hydrophilic binder and on which the enzyme is immobilized is used for at least one of the positive electrode and the negative electrode. Ketjen black or the like is used for carbon particles, and ethyl cellulose or the like is used for the binder.
展开▼