Nonlinear optical microscopy and nonlinear optical microscopy

摘要

In the nonlinear optical microscope to measure the signal light generated from the nonlinear optical process by plurality of pump lights, located modulated at a predetermined frequency centroid of the focal point of the excitation light, a plurality of frequency components corresponding to the modulation frequency of the signal light extract. Extracting frequency components, can be an even multiple of the modulation frequency is preferred. Also, as the position modulation method, or a method of moving spirally or straight in a plane perpendicular to the optical axis, the focal point the center of gravity, or moved linearly in the optical axis direction.