REGULATION OF EUKARYOTIC GENE EXPRESSION BY RIBOSOMAL READING-FRAME SWITCH EFFICIENCY CONTROL VIA REGULATORY ELEMENT UPSTREAM OF THE FRAMESHIFTING SITE

摘要

A method of regulating gene expression in eukaryote includes administrating a ligand-sensing RNA element to regulate the formation of regulatory hairpin upstream of programmed ribosomal frameshifting (PRF) site. Further, a method of regulating ribosome frameshifting efficiency in the protein translation of a eukaryotic cell includes contacting the eukaryotic cell with a molecule to control the upstream programmed ribosomal frameshifting (PRF) regulatory duplex element formation, which does not involve messenger RNA degradation by RNase H or RNAi.