Randomized Humanized Antibody Libraries Engineered with Sequence and Insert Diversity

摘要

A phage display library is formed by a plurality of recombinant phages; each of the plurality of recombinant phages comprising an M13-derived expression vector; the M13-derived expression vector comprising a polynucleotide sequence encoding a randomized peptide, wherein the randomized peptide is a variable region of a human IgG1 heavy chain; the randomized peptide is expressed in absence of an immunoglobulin light chain protein on an outer surface of a recombinant phage of the phage display antibody library; and a protein product expressed by a recombinant phage of the phage display antibody library fuses with a human immunoglobulin constant region to form a full human IgG1 heavy chain molecule. The phage display library can be utilized to identify candidate antibodies that can bind to receptor Axl and other receptors.