Method for increasing yield of total flavonoids in Ganoderma lucidum mycelium

摘要

A method for increasing the yield of total flavonoids in Ganoderma lucidum mycelium by an expansin comprises: (1) inoculating Ganoderma lucidum into the PD liquid fermentation medium, activating the culturing of the strains and obtaining a seed solution; (2) inoculating the seed solution into a liquid fermentation medium, culturing and adding the expansin solution, then further culturing, isolating and obtaining Ganoderma lucidum mycelium; (3) extracting flavonoids from the Ganoderma lucidum mycelium and obtaining total flavonoids. A plant expansin is used for the liquid fermentation production of flavonoids ingredients of Ganoderma lucidum, and the fermentation process and extraction method are optimized to increase greatly the yield of total flavonoids in Ganoderma lucidum.