Compositions and Methods for controlling Leptinotarsa

摘要

In addition, Methods to provide plants with resistance to these pests. Also described polynucleotide constructs and recombinant DNA molecules and compositions useful in such methods, such as topical insecticide sprays containing RNA double chain Insecticides and solanaceous plants with improved resistance to infestation of LEP Tinotarsa spp.It also describes methods to Select target genes for Silencing mediated by RNAi and Control of Leptinotarsa spp.Claim 1: a method to control the infestation of a plant species Leptinotarsa comprises (a) contacting said with a polinucleu00f3tido Leptinotarsa species comprising a Nucleotide sequence that is complementary to at least 21 nucleotides of a contiguous Target Gene has a Nucleotide sequence which is selected from the group consisting of: SEQ ID no. 730, SEQ ID no. 807, SEQ ID no. 1 - SEQ ID no: 725 - 726, 729, SEQ ID no. 731 - SEQ ID no. 806, 808 - 830, and SEQ ID no. 1087 - 1094, or RNA transcribed from Said Target Gene; or (b) provide in the diet of the species a polinucleu00f3tido Leptinotarsa comprising a sequence of nucleotides At least 21 nucleotide is complementary to a contiguous geneDiana has a Nucleotide sequence which is selected from the group consisting of: SEQ ID no. 730, SEQ ID no. 807, SEQ ID no. 1 - SEQ ID no. 725 - 726, 729, SEQ ID no. 731 - SEQ ID no. 806, 808 - 830, and SEQ ID no. 1087 - 1094Or an RNA transcribed from Said Target Gene; or (c) cause mortality or weakening of the larvae of this species in the diet of Leptinotarsa providing such a polinucleu00f3tido larvae at least comprises at least a Silencing Element comprising 21 nucleotides adjacent Q EU are complementary to a Target Gene has a Nucleotide sequence which is selected from the gruPO is: SEQ ID no. 730, SEQ ID no. 807, SEQ ID no. 1 - SEQ ID no. 725 - 726, 729, SEQ ID no. 731 - SEQ ID no. 806, 808 - 830, and SEQ ID no. 1087 - 1094Or an RNA transcribed from Said Target Gene; or (d) topically applied to the plant a composition comprising at least one polinucleu00f3tido comprising a Nucleotide sequence that is complementary to at least 21 nucleotides adjacent to a Target Gene has a Nucleotide sequence That is selected from the group consisting of: SEQ ID no. 730, SEQ ID no. 807, SEQ ID no. 1 - 725SEQ ID no. 726 - 729, SEQ ID no. 731 - SEQ ID no. 806, 808 - 830, and SEQ ID no. 1087 - 1094, or RNA transcribed from Said Target Gene; or (e) topically applied to the plant a composition comprising at least one polinucleu00f3tido so that an effective amount of said polinucleu00f3tido is Ingested by the species Leptinotarsa that feeds on the Plant,The polinucleu00f3tido comprises at least 21 contiguous nucleotides that are complementary to a Target Gene that has a sequence.Nucleotides that is selected from the group consisting of: SEQ ID no. 730, SEQ ID no. 807, SEQ ID no. 1 - SEQ ID no. 725 - 726, 729, SEQ ID no. 731 - SEQ ID no. 806, 808 - 830, and SEQ ID no. 1087 - 1094Or an RNA transcribed from Said Target Gene; or (f) express in the plant at least a polinucleu00f3tido comprising at least one segment that is identical or complementary to at least 21 contiguous nucleotides of DNA that has a sequence that is selected from the group consisting of: SEQ ID no. 730 SEQ ID no. 807, SEQ ID no. 1 - SEQ ID no. 725 - 726, 729, SEQ ID no. 731 - SEQ ID no. 806, 808 - 830And SEQ ID no. 1087 - 1094; or (g) to provide the plant at least a polinucleu00f3tido comprising at least one segment that is identical or complementary to at least 21 nucleotides adjacent to a Target Gene or RNA transcribed from the Gene Target, where the target Gene.Select the grupou203a consists in: the group of genes identified in the sequences of target Gene or RNA transcribed from Said Target Gene; or (H) contacting Said Leptinotarsa species with an effective amount of a double stranded rna, which is complementary to a Chain of at least 2 1 contiguous nucleotides of a gene encoding a Ribosomal Protein.Where It induces RNA interference and mortality occurs; or (i) contacting said with a polinucleu00f3tido Leptinotarsa species comprising at least one segment that is identical or complementary to at least 21 nucleotides adjacent to a Target Gene that is selected from the Group consis You: the group of genes identified in the sequences of target Gene or RNA transcribed from the geneDiana.