Method and kit for the identification and molecular differentiation of Salmonella pullorum and Salmonella gallinarum

摘要

Method and kit Pope identification and molecular differentiation of Salmonella pullorum and Salmonella gallinarum.To this invenu00e7u00e3op refers to a method for the identification and molecular differentiation of Samonella pullorom and Samonella gallinarum comprising the steps of identifying segments of DNA targets; the construction of the oligonucleotides primers; extraction of bacterial DNA; and reau00e7u00e3 The polymerase chain (PCR).In addition, the present invention relates to a diagnostic kit for detecting avian salmonellosis, more specifically the fowl typhoid (caused by S. gallinarum) and Pullorosis (caused by S. pullorum). The kit contains primers, Taq DNA polymerase, desoxinucleotu00eddeos, buffer solution with KCl added mgc12 and stabilizers for polymerase chain reaction (PCR).This is a duplex PCR (two pairs of primers) capable of amplifying specific manner in regions of the genome of Salmonella gallinarum and Samonella pullorum.