Methods to test the activity of cellulase and for separating a solution of enzyme and micro organisms producing cellulase strains, and.

摘要

The present invention is a method to test the activity of cellulase.Including: a process (a) to prepare two or more solutions of substrate that have a similar measure absorbance in a wavelength identical and in which cellulose is dispersed in a similar concentration and measuring the absorbance of each of the solutions of the substrate; a process (b) Respectively add different types of solutions of enzyme to substrate and conducting a reaction solutions.Enzyme under the same conditions; a process (c) to measure the absorbance of each of the solutions of the substrate after the enzymatic reaction; a process (d) to calculate the values of decrease of absorbance of the solutions of the substrate before and after the enzymatic reaction and a process; The (and) to test the activity of Cellulase of the solutions of enzyme on the basis of the decrease of absorbance.In the test of the process (E), stipulates that the solutions of enzyme have higher activity of cellulase to the extent that the values of decrease of absorbance are larger.