A METHOD FOR ERROR-PRONE DNA POLYMERASE MEDIATED IN VIVO TARGETED RANDOM MUTAGENESIS FOR IMPROVED TRAITS OF INTEREST

摘要

The present disclosure provides a method for in vivo targeted random mutagenesis in an organism using error prone DNA polymerase. The disclosure also provides a microorganism that includes a nucleic acid construct encoding an error prone DNA polymerase for in vivo targeted random mutagenesis. The present disclosure also describes a kit for in vivo targeted mutagenesis in an organism.