GENERATION OF NEWCASTLE DISEASE VIRUS STRAIN R2B FROM CDNAS BY REVERSE GENETICS TECHNOLOGY

摘要

The present invention relates to an isolated recombinant polynucleotide of NDV R2B strain comprising following specific mutations relative to the NDV sequence ID NO. 1: i) addition of PacI site between nucleotides 3075 and 3082 by replacing TCATTACT with TTAATTAA ii) addition of MluI site between nucleotides 13042 and 13047 by replacing ACGTGT with ACGCGT iii) deletion of NheI site between nucleotides 14527 and 14531 by replacing GCTAGCwith GCTTGC iv) deletion of AvrII site between nucleotides 14863 and 14867 by replacing CCTAGGwith CCTGGG.